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Culture of Animal Cells: A Manual of Basic Technique
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Binding: Hardcover
Dewey Decimal Number: 571.6381
EAN: 9780471453291
Edition: 5
ISBN: 0471453293
Label: Wiley-Liss
Manufacturer: Wiley-Liss
Number Of Items: 1
Number Of Pages: 672
Publication Date: July 29, 2005
Publisher: Wiley-Liss
Studio: Wiley-Liss
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Editorial Review:
The most complete resource on the techniques, equipment,principles, and practices of animal cell culture
Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.
The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites
Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
Customer Reviews
Average Rating: 
Rating: - Fantastic book!
Based on protocols in this book, I devised methods to complete my own experiments. I found the insights contained in the book completely indispensable. The range of topics it contains and the detail with which Freshney explains each point made the process of designing conditions for experiments rational and approachable. I highly recommend this book.
Rating: - useful book
There are not a lot of glossy pretty pictures, which is a good thing for a no-nonsense instruction manual that gets the point across with what pictures it did have. I found it to be a very good complement to my cell culture class. Everything I learned in the lab was put into context nicely.
Rating: - Save on Textbook
I purchased this book with a description of like new. I would say this is new. The previous person who used this did not even break in the binder. This is an excellent reference book, I have enjoyed reading the text up to this point.
Rating: - Best Basic Book on Tisssue Culture
For many years Freshney's Culture of Animal Cells has been the gold standard of tissue culture reference books. This new edition adds and updates many techniques and continues to be the best book in its area. I use it extensively both for my own research and in teaching classes on tissue culture.
Rating: - The best book on cell culture
Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, ... Read More
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